What is CD45 staining?
CD45 (Leucocyte Common Antigen) Antibody Staining Protocol for Immunohistochemistry. Description: CD45 is a transmembrane glycoprotein expressed on most nucleated cells of haematopoietic origin. These isoforms are recognized by CD45RA, CD45RB, CD45RC and CD45R0 antibodies.
What does CD45 antibody Stain for?
The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This antibody is specific for mouse CD45 and suitable for immunohistochemical staining of Zinc-fixed and formalin fixed paraffin sections as well as acetone-fixed, frozen sections.
What is CD45 test?
CD45 (Leukocyte Common Antigen), IHC with Interpretation – This immunoassay is a first-line screening test to evaluate neoplasms of unknown origin and to help determine whether a cell is derived from a hematopoietic origin. In benign tissue or cell blocks, CD45 can be used as a marker of inflammatory cells.
What is CD45 FITC?
CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. CD45 plays a key role in TCR and BCR signal transduction.
What cells are CD45+?
CD45 antigen (leukocyte common antigen), a unique and ubiquitous membrane glycoprotein with a molecular mass of about 200 kDa, is expressed on almost all hematopoietic cells except for mature erythrocytes. However, the biological function of this glycoprotein still remains to be resolved.
Which cells are CD45 +?
CD45 (lymphocyte common antigen) is a receptor-linked protein tyrosine phosphatase that is expressed on all leucocytes, and which plays a crucial role in the function of these cells.
What cells are CD45 positive?
CD45 is a transmembrane protein tyrosine phosphatase located on most haematopoietic cells. It has several isoforms, and haematopoietic cells express one or more of the isoforms—CD45RO, CD45RA and CD45RB. CD45 immunoreactivity is recognised to be highly specific for non–Hodgkin’s lymphomas.
How is CD45 activated?
The transmembrane protein tyrosine phosphatase CD45 is critical for activation through the T cell receptor (TCR) after recognition of agonist peptide presented by the appropriate MHC molecule (3).
Are B cells CD45+?
Analysis of CD45 by flow cytometry revealed that unstimulated and stimulated B cells expressed homogeneous amounts of total CD45 but that stimulation with IL-5 resulted in a CD44hi, hyaluronate-adherent subpopulation of activated B cells that expressed a markedly altered pattern of expression of exon-specific CD45R or …
Is CD45 a phosphatase?
CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu Rev Immunol. 1994;12:85-116.
Where is CD45 found?
hematopoietic cells
CD45 is a type I transmembrane molecule found on the surface of all nucleated hematopoietic cells and their precursors2 (Fig. 1). In T and B cells it has been estimated that CD45 comprises up to 10% of the cell surface area1.
Do all white blood cells have CD45?
CD45 was initially characterized as a leukocyte common antigen and can be found on all hematopoietic cells with exception of platelets and red blood cells.
What is the antibody staining protocol for CD45?
CD45 Antibody Staining Protocol for Immunohistochemistry Description: This antibody reacts with both alloantigens and all isoforms of the CD45 leukocyte common antigen (LCA), also known as Ly-5 or T200, found on all cells of hematopoietic origin, except erythrocytes.
What kind of blood cells are CD45 +?
Hematopoietic cells, including basophils, granulocytes, lymphocytes, macrophages / histiocytes, mast cells, monocytes and plasma cells; NOT mature red blood cells and their immediate progenitors, platelets or megakaryocytes Note that infiltrating lymphocytes in any disorder are typically CD45+, and they must be distinguished from tumor cells
What happens when a CD45 mutation is lost?
Loss of CD45 mutations cause severe combined immunodeficiency-autosomal recessive, T cell negative, B cell positive and NK cell positive (OMIM #608971); patients have a defect in B/T cell development, lymphopenia and deficiency in humoral and cell-mediated immunity
What is the function of the CD45 glycoprotein?
CD45 is a transmembrane glycoprotein which is expressed at high levels on the cell surface, and its presence distinguishes leukocytes from non-hematopoietic cells. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.