What is the emission wavelength of FITC?
475 to 650 nm
FITC emits fluorescence from 475 to 650 nm, peaking at 525 nm, which falls in the green spectrum.
Are fluorescein and FITC the same?
FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm.
Is FITC a GFP?
Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP.
What makes GFP glow?
Scientists knew that GFP glows because three of its amino acids form a fluorophore, a chemical group that absorbs and emits light.
Is FITC a 488?
Molecular Probes™ Alexa Fluor® 488 dye—with nearly identical spectral properties and quantum yield as fluorescein (FITC)—produces brighter, more photostable conjugates that are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection.
Is FITC a Fluorochrome?
FITC (Ex-Max 494 nm/Em-Max 520 nm): Fluorescein isothiocyanate – has a very high efficiency of energy transfer from absorbed to emitted light and is one of the most commonly used fluorochromes.
What is the absorption efficiency of FITC fluorophore?
The green spectrum is the absorption profile of fluorescein isothiocyanate ( FITC ), which has an absorption maximum of 495 nanometers. Excitation of the FITC fluorophore at 488 nanometers using an argon-ion laser produces an emission efficiency of approximately 87 percent.
Is the spectrum of Alexa Fluor 488 identical to FITC?
Alexa Fluor 488 has a spectrum almost identical to that of fluorescein isothiocyanate (FITC), but with extraordinary photostability. Because of this photostability, it has become a choice for fluorescent microscopy applications and has become popular in cytometry applications.
What happens when fluorochrome is excited by a neon laser?
When excited by light from a helium-neon laser, the excited fluorochrome (APC) is able to transfer its fluorescent energy to the cyanine molecule, which then fluoresces at a longer wavelength. The resulting fluorescent emission maximum is in the deep red at approximately 767 nanometers.
Why is the shape of the emission spectrum always the same?
Since the emission of fluorescence always takes place from the lowest vibrational level of the first excited state, the shape of the emission spectrum is always the same, despite changing the wavelength of exciting light. A plot of emission against wavelength for any given excitation wavelength is known as the emission spectrum.