What is smart-seq?
Smart-Seq and Smart-Seq2: Switch Mechanism at the 5′ End of RNA Templates Smart-Seq was developed as a single-cell sequencing protocol with improved read coverage across transcripts. Complete coverage across the genome allows the detection of alternative transcript isoforms and SNPs.
How does smart-seq work?
Smart-Seq: Cells are lysed, and the RNA is hybridized to an oligo(dT)-containing primer. An oligonucleotide primer is hybridized to the poly(C) overhang and used to synthesize the second strand. Full-length cDNAs are PCR-amplified to obtain nanogram amounts of DNA. The PCR products are purified for sequencing.
Does RNA-Seq use cDNA?
Summary of RNA-Seq. Within the organism, genes are transcribed and (in an eukaryotic organism) spliced to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods.
What is TruSeq RNA?
TruSeq Stranded Total RNA A robust, highly scalable whole-transcriptome analysis (RNA-Seq) solution for a variety of species and sample types, including human, mouse, and formalin-fixed, paraffin-embedded (FFPE) tissue.
How expensive is single cell RNA seq?
The Genomics CoLab carries out all standard 10x Genomics workflows for single cell RNA-seq or ATAC-seq….Library preparation.
| Assay Type | Cost per well |
|---|---|
| 3’/5′ Gene Expression + FB | $1650 |
| 3’/5′ Gene Expression + TCR or BCR + FB | $1730 |
| Single Cell ATAC-seq | $1610 |
| Combined Single Cell RNA-seq and ATAC-seq | TBD |
What does ATAC-seq measure?
The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome. By sequencing regions of open chromatin, ATAC-Seq can help you uncover how chromatin packaging and other factors affect gene expression.
What does ATAC seq measure?
How does bulk RNA seq work?
Bulk RNA-Seq experiments provide a view of gene expression of an entire sample. This is done by dissociating the sample into individual single cells, identifying the cell types, and measuring the expression products of each cell.
What can RNA-Seq tell us?
RNA-seq can tell us which genes are turned on in a cell, what their level of transcription is, and at what times they are activated or shut off. This allows scientists to understand the biology of a cell more deeply and assess changes that may indicate disease.
What is an RNA-seq library?
RNA sequencing (RNA-seq) is a tool used to study the transcriptome – the total RNA molecules present in one or a collection of cells, including protein coding RNAs (mRNA) and regulatory or non-coding RNAs (miRNA, tRNA etc.).
How is smart seq used to generate cDNA?
The SMART-Seq Single Cell Kit is designed to generate high-quality, full-length cDNA directly from single cells. It has been validated with 2 pg of total RNA input and with single cells known to have low RNA content (e.g., peripheral blood mononuclear cells). This kit supports up to 12 reactions.
Which is the best smart seq single cell KIT?
Start from our: SMART-Seq Single Cell Kit to generate cDNA from single cells, SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing to generate cDNA from multiple intact cells (up to 1,000 cells) or 10 pg–10 ng of total RNA, or SMART-Seq HT Kit for automation-friendly generation of cDNA from single cells or 10 pg–1 ng of total RNA.
How does the Smart seq V4 plus Kit work?
The SMART-Seq v4 Kit (SSv4) and the SMART-Seq v4 PLUS Kit (SSv4 PLUS) are the fourth generation of our SMARTer ultra-low solutions and our most sensitive kits for ultra-low inputs. These kits use oligo (dT) priming to generate high-quality, full-length cDNA directly from multiple intact cells (up to 1,000 cells) or 10 pg–10 ng of total RNA.
Which is better smart seq or smart-seq2 for RNA sequencing?
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing has higher sensitivity and reproducibility, meaning more genes are identified from libraries produced with this kit, and significantly lower background than the SMART-Seq2 method.