What is A375 cell line?
A375 (ATCC® CRL-1619™) is a human melanoma cell line initiated through explant culture of a solid tumor from a 54-year-old female. The cells are adherent with an epithelial morphology. The cells form tumors following implantation into immunocompromised mice.
Is melanoma genetic?
Few people inherit melanoma genes About 10% of melanomas are caused by a gene mutation (change) that passes from one generation to the next. Most people get melanoma for other reasons. The sun, tanning beds, and tanning lamps give off ultraviolet (UV) rays. These rays are known to damage our skin.
How does melanoma affect the cell cycle?
The body can often correct mutations, but if it doesn’t, the mutation creates an abnormal protein or prevents one from forming, which in turn causes a cell to multiply uncontrollably and become cancerous. Melanoma develops when melanocytes (the cells that produce pigment), mutate, multiply, and become cancerous.
What is melanoma?
Melanoma is a type of cancer that begins in melanocytes (cells that make the pigment melanin). Below are photos of melanoma that formed on the skin. Melanoma can also start in the eye, the intestines, or other areas of the body with pigmented tissues.
What is B16F10?
B16F10 (ATCC® CCL-6475™) is a murine melanoma cell line from a C57BL/6J mouse. It is a subclone of the B16 tumor line, generated by injecting mice with B16 tumor cells, collecting and culturing secondary tumor growths, and injecting them into fresh mice, a total of 10 times.
Who is more prone to melanoma?
Melanoma is more likely to occur in older people, but it is also found in younger people. In fact, melanoma is one of the most common cancers in people younger than 30 (especially younger women).
Does melanoma only come from moles?
Most melanoma does not start in a preexisting mole. Melanoma can develop in a preexisting mole, says Dr. Marghoob, but nearly 70% of skin melanomas do not. Rather, they occur in normal skin. “Moles themselves are not cancerous, and it is extremely rare for a mole to transform into a melanoma,” says Dr.
What is melanoma in situ?
(MEH-luh-NOH-muh in SY-too) Abnormal melanocytes (cells that make melanin, the pigment that gives skin its natural color) are found in the epidermis (outer layer of the skin). These abnormal melanocytes may become cancer and spread into nearby normal tissue. Also called stage 0 melanoma.
What are the differences between A375 and δE2 melanoma cells?
The differences found at the molecular level between A375 and A375 ΔE2 cells are consistent with their notable differences in cell motility, percentage of amaeboid cells, invasiveness and cell proliferation.
Which is the best transfection host for A375?
Applications This cell line is a suitable transfection host This cell line is also the ideal control for NRAS mutant A375 isogenic cell line ATCC CRL 1619IG 2 Host Homo sapiens human This cell line is a suitable transfection host.
What is the role of Ladon in melanoma metastasis?
Figure Legend Snippet: The comparison of A375 and A375ΔE2 cells supports a role for LADON in melanoma metastasis. In melanoma A375 cells, the LADON transcript (in blue) promotes the transition from a proliferative cell identity to a less proliferative and more invasive cell identity.
What is the role of cytokeratin in melanoma?
Occasional reports indicated cytokeratin (CK) protein expression (mainly by immunohistochemistry) in malignant melanoma (MM) and suggested an association with unfavorable clinical parameters. However, the mRNA expression of CK and its clinicopathologic significance in MM has not been specifically ev …