What is API 20 NE?
The API 20 NE (BioMérieux) is a commercial biochemical identification system for gram-negative non-Enterobacteriaceae, such as Pasteurella. The test strip contains 20 miniature biochemical tests that are inoculated with a sample of a bacterial colony.
How do you use API 20 E?
Take the API20E Biochemical Test Strip which contains dehydrated bacterial media/bio-chemical reagents in 20 separate compartments. Using a pasteur pipette, fill up (up to the brim) the compartments with the bacterial suspension. Add sterile oil into the ADH, LDC, ODC, H2S and URE compartments.
How does an Enterotube work?
The Enterotube™ II is inoculated by touching the wire to a well isolated colony from a Petri plate. The wire is pulled and rotated from the other end to inoculate all the compartments and pushed all the way back in to reinoculate the compartments.
What is API biomerieux?
Easy and reliable identification of microorganisms. API® makes microbial identification easy and reliable. Combining a simple standardized method with extensive and robust online microbial ID knowledge bases, API® is quickly and easily implemented into any microbiology laboratory.
What is API system in microbiology?
The analytical profile index or API is a classification of bacteria based on biochemical tests, allowing fast identification. This system is developed for quick identification of clinically relevant bacteria.
Why we are doing API testing?
The purpose of API Testing is to check the functionality, reliability, performance, and security of the programming interfaces. In API Testing, instead of using standard user inputs(keyboard) and outputs, you use software to send calls to the API, get output, and note down the system’s response.
How do I use API 20e?
Add sterile oil into the ADH, LDC, ODC, H2S and URE compartments. Put some drops of water in the tray and put the API Test strip and close the tray. Mark the tray with identification number (Patient ID or Organism ID), date and your initials. Incubate the tray at 37oC for 18 to 24 hours.
What is oxidase reagent?
The oxidase reagent contains a chromogenic reducing agent, which is a compound that changes color when it becomes oxidized. If the test organism produces cytochrome oxidase, the oxidase reagent will turn blue or purple within 15 seconds.
What is API test strip?
API test strips consists of wells containing dehydrated substrates to detect enzymatic activity, usually related to fermentation of carbohydrate or catabolism of proteins or amino acids by the inoculated organisms. A bacterial suspension is used to rehydrate each of the wells and the strips are incubated.
What is an API test kit?
API PH Test Kit (Low Range): This is the test for PH levels from 6.0 to 7.6. It has enough solution for around 250 tests. API PH Test Kit (High Range): This is the test for PH levels from 7.4 to 8.8. It has enough solution for around 160 tests.
What is API in microbiology?
API stands for Analytical Profile Index (microbiology) Suggest new definition. This definition appears very frequently and is found in the following Acronym Finder categories: Science, medicine, engineering, etc.