What is the independent variable in the enzyme lab?

What is the independent variable in the enzyme lab?

The independent variable is the concentration of enzyme, the concentration of the substrate, the temperature, and the addition of an inhibitor. Introduction: Enzymes are proteins that carry out chemical reactions.

What factors affect catalase activity?

The rate at which an enzyme works is influenced by several factors including the concentration of substrate (hydrogen peroxide in the case of catalase), temperature, pH, salt concentration and the presence of inhibitors or activators. Every enzyme has an optimal range for each of these factors.

How do you investigate catalase?

Catalase

1. The activity of catalase can be measured when in a solution because bubbles of oxygen gas are produced – visible as foam.
2. The greater the height of the foam in the test tube, the greater the catalase enzyme activity.

What is the independent variable in this experiment What is the dependent variable?

The independent and dependent variables are the two key variables in a science experiment. The independent variable is the one the experimenter controls. The dependent variable is the variable that changes in response to the independent variable. The two variables may be related by cause and effect.

What is the catalase reaction used for?

This test is used to identify organisms that produce the enzyme, catalase. This enzyme detoxifies hydrogen peroxide by breaking it down into water and oxygen gas. The bubbles resulting from production of oxygen gas clearly indicate a catalase positive result.

How does the catalase and hydrogen peroxide experiment work?

Catalase and Hydrogen Peroxide Experiment. Enzymes are molecules that enable the chemical reactions that occur in all living things on earth. In this catalase and hydrogen peroxide experiment, we will discover how enzymes act as catalysts by causing chemical reactions to occur more quickly within living things.

What’s the easiest way to isolate catalase enzyme?

But it makes isolating the catalase enzyme very inexpensive and easy if you want to run a simple experiment. The following is a simple enzyme experiment anyone can run. 1. Cut up a potato and mash it. Do not cook it, cooking will break down the enzyme so it won’t work. 2. Place the mashed potato in a test tube or other small container. 3.

How is the rate of reaction of catalase calculated?

The enzyme, Catalase can be obtained from assaccharomyces cerevisiae and in this experiment Catalase will be obtained from assaccharomyces cerevisiae. Oxygen is a product of the break down of Hydrogen Peroxide and in this experiment, the rate of reaction will be calculated by measuring the Oxygen given off with a Vernier Oxygen Probe.

What kind of tissues contain the enzyme catalase?

The tubes contain extracts from beef muscle, kidney, and liver from left to right. In a post awhile back we discussed the enzyme catalase and its presence in animal tissues such as liver, kidney, and muscle.