What is radio HPLC?
Applications of Radio-HPLC Pharmaceutical Analysis: Radio-HPLC is a key technology for the separation and quantitation of radiolabeled drugs and putative metabolites in drug development.
What is universal detector in HPLC?
A universal detector is defined as the one which ‘can respond to every component in the column effluent except the mobile phase’2. In contrast, selective detectors are defined as ‘detectors which respond to a related group of sample components in the column effluent’.
What is the purpose of the detector in HPLC?
HPLC detectors are used in the detection of the solute present in the eluent coming from the HPLC column. They are capable of determining the identity and concentration of eluting compounds in the mobile phase.
What are detectors used for?
The interactions of alpha, beta, and gamma radiations with matter produce positively charged ions and electrons. Radiation detectors are devices that measure this ionization and produce an observable output. Early detectors used photographic plates to detect “tracks” left by nuclear interactions.
What is the difference between UV and PDA detector?
PDA detects an entire spectrum simultaneously. UV and VIS detectors visualize the obtained result in two dimensions (light intensity and time), but PDA adds the third dimension (wavelength). This is convenient to determine the most suitable wavelength without repeating analyses.
What is the principle involved in detector?
Detection principle. This detection technique requires discriminating upward going muons against the much higher flux of downward atmospheric muons (see figure below). To simplify the discrimination, the detector is installed in a deep site where a layer of water or ice would shield it.
Why do you need a radio HPLC detector?
Development and QC of Radiochemicals: Radiochemicals are only as good as the care taken in each preparation step. Strict regulations have to be met during production. Radiochemical purity analysis using Radio HPLC is often required to verify the quality of the produced radiochemicals.
How is radio HPLC used in pharmaceutical analysis?
Pharmaceutical Analysis: Radio-HPLC is a key technology for the separation and quantitation of radiolabeled drugs and putative metabolites in drug development. Environmental Analysis: A wide variety of contaminants from sources such as industrial waste, landfill sites, pesticides and pharmaceutical drugs can make their way into the environment.
Which is radio HPLC detector from Berthold technologies?
Whatever you need, there is a radio HPLC detector from Berthold Technologies to take your work in radiochromatography to the next level. The NaI detector option for the HERM combines an extremely sensitive NaI detector with a compact shielding. Even low energy gamma isotopes like I-125 can be detected with high efficiency
Are there universal detectors for high pressure chromatography?
There are different types of chromatography; in the case of High-Pressure Liquid Chromatography (HPLC), the mobile phase is a liquid at high pressure (typically 400 bar). In order to measure the separation of the mixture, its components have to be detectable in some way. There is no universal detector that can monitor all compounds.