Does Clostridium perfringens grow on blood agar?
At least 4 different colony form of Clostridium perfringens on the surface of yeast extract blood agar after overnight incubation were demonstrated. A: glossy colonies with entire margins, mostly surrounded by narrow zones of complete hemolysis and a much wider zone of incomplete hemolysis (colonies typical for C.
What agar does Clostridium perfringens grow on?
TSC agar
C. perfringens is provisionally identified as a nonmotile, Gram-positive bacillus which produces black colonies in TSC agar, reduces nitrates to nitrites, produces acid and gas from lactose, and liquefies gelatin within 48 h.
How do you test for Clostridium perfringens?
Although primary isolation on tryptose sulfite cycloserine (TSC) agar takes 24 h, the standard method of confirmation for presumptive C. perfringens can take up to 72 h (1, 13). TSC agar incorporates sodium metabisulfite and ferric ammonium citrate as an indicator for sulfite reduction.
What is the morphology of Clostridium perfringens?
Morphology of Clostridium perfringens Clostridium perfringens is large rectangular gram-positive bacilli with rounded or truncated ends. It is pleomorphic with straight or curved rods. Size is about 3-8 µm X 0.4-1.2 µm. It is a capsulated, non-motile, and non-flagellated pathogenic bacteria.
What kind of media would you use to cultivate a Clostridium species?
BSM agar is a selective and differential media used for the presumptive identification of Clostridium botulinum from food or fecal samples. BSM contains cycloserine, sulfamethoxazole, and trimethoprim as selective inhibitory agents.
Which media is used for Clostridium?
A selective and differential agar medium that contains cycloserine, cefoxitin, fructose, and egg yolk (CCFA) was developed to facilitate the isolation of C. difficile from fecal specimens.
What media is used for Clostridium perfringens?
An improved selective medium, Tryptose-sulfite-cycloserine (TSC) agar, for the enumeration of Clostridium perfringens is described. It consists of the same basal medium as Shahidi-Ferguson-perfringens (SFP) agar, but with 400 μg of D-cycloserine per ml substituted for the kanamycin and polymyxin.
What are the virulence factors of C perfringens?
The mechanism of action of these factors usually falls into one of three groups. Some of these virulence factors, such as the alpha toxin, which is phospholipase C, and the kappa toxin, which is a collagenase, are enzymes that hydrolyze substances essential to the integrity of membranes or other body structures.
Is C Sporogenes an Anaerobe?
Clostridium sporogenes is an anaerobic, gram-positive bacillus that comprises a part of the normal intestinal flora.
Can C Sporogenes ferment mannitol?
Clostridium difficile ATCC 43255 fermented less than 10% of the mannitol in a medium at pH 7; however, when the initial pH of the medium was adjusted to 8.5 or 9, about 80% of the mannitol was fermented.
How is a swab of Clostridium perfringens inoculated?
The swab is inoculated on Pus plates: Anaerobic blood agar, Blood agar, MacConkey, Chocolate agar and Thioglycolate broth. The anaerobic blood is incubated anaerobically while the other plates are incubated capnophilically at 37oC for 24 to 48 hours.
What does Clostridium perfringens look like on Gram stain?
C. perfringens is nonmotile and generally has a distinctive “boxcar” appearance on Gram stain of clinical material or subcultures. On blood agar, the colonies are typically surrounded by a “double zone of hemolysis”: an inner zone of complete hemolysis that is due to θ-toxin and a larger outer zone of incomplete hemolysis that is due to α-toxin.
How long to incubate Clostridium perfringens in a jar?
C. perfringens is a short, thick, Gram-positive bacillus. If there is evidence of contamination, streak contaminated culture (s) on TSC agar containing egg yolk and incubate in anaerobic jar 24 h at 35°C.
What kind of media is used to grow Clostridium perfringens?
⇒ Special requirements – Clostridium perfringens or C. welchii have complex nutritional requirements and readily grow in a media containing Blood or Egg, commonly Blood Agar medium & Robertson’s Cooked meat broth medium is used for the cultivation of Clostridium perfringens in Laboratory.