What color is Cy3?
Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
Is Cy5 a protein?
The Cy5-labeled protein has excitation and emission wavelengths at 650 nm and 670 nm respectively, and can be directly used for downstream applications including ELISA, western blot, Immunohistochemistry, Immunofluorescence, and FACS, etc.
Is Cy5 far red?
Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines.
What is FAM in DNA?
Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide synthesis and molecular biology. Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons.
What are cy7 dyes used for in vivo imaging?
Cy®7 is useful for in vivo imaging when tissues are observed in the near-infrared region. Cy® cyanine dyes are registered trademarks of GE Healthcare.
Where did the name Cy3 and Cy5 come from?
The Cy3 and Cy5 nomenclature was first proposed by Ernst, et al. in 1989, and is non-standard since it gives no hint of their chemical structures. In the original paper the number designated the count of the methines (as shown), and the side chains were unspecified.
What is the absorption wavelength of CY7 dye?
Cy®7 is a near-infrared (NIR) fluorophore commonly used in applications such as immunolabeling, fluorescence microscopy, and flow cytometry. The dye has an absorption wavelength that peaks around 743 nm, and an emission maximum around 767 nm.
What kind of dye is c7-cyanine dye?
Cy7 dye is a C7-cyanine dye having differentially-substituted 2-indolyl units at each end. It has a role as a fluorochrome. It derives from a C7-indocyanine.