What is Qubit DNA?
Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more specific than UV absorbance. More sensitive than UV absorbance, detecting as little as 10 pg/μl of DNA. Uses as little as 1 μL of sample, even with very dilute samples.
How long is qubit solution good for?
To allow the Qubit® assay to reach optimal fluorescence, incubate the tubes for the DNA and RNA assays for 2 minutes after mixing the sample or standard with the working solution. After this incubation period, the fluorescence signal is stable for 3 hours at room temperature.
What does a qubit do?
The Qubit fluorometer is a lab instrument developed and distributed by Invitrogen (now part of Thermo Fisher) that, among other applications, is used for the quantification of DNA, RNA, and protein.
How does qubit DNA work?
The Qubit fluorometer uses fluorescent dyes that emit signals only when bound to the specific target molecules (DNA or RNA) even in the presence of free nucleotides, degraded nucleic acids, or protein contaminants.
What does a Qubit do?
What dye is used in Qubit?
The evolution of dye selection has been discussed in detail, ranging from Ethidium Bromide (commonly used as a fluorescent tag for gel electrophoresis), to the dye chosen here for DNA quantification in QUBIT 4.0, called PicoGreen (ultra sensitive dye which is used for ds – DNA quantification in QUBIT 4.0).
How does Qubit DNA work?
Each dye is specific for one type of molecule (DNA, RNA or protein). The Qubit fluorometer picks this fluorescence signal up and converts it into a DNA concentration measurement by referring to DNA probes of known concentration. It then uses this relationship to calculate the concentration of a sample.
What Dye does Qubit use?
Can you use PCR tubes for qubit?
Only thin-wall, clear 0.5 mL PCR tubes are appropriate for use in the Qubit® 2.0 Fluorometer.
How does the qubit dsDNA BR assay kit work?
The Qubit dsDNA BR (Broad-Range) Assay Kits make DNA quantitation easy and accurate. The kits include concentrated assay reagent, dilution buffer, and prediluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume from 1–20 μL is acceptable), then read the concentration using the Qubit
How to calculate the concentration of qubit dsDNA HS?
The Qubit® 2.0 Fluorometer gives values for the Qubit® dsDNA HS Assay in ng/mL. This value corresponds to the concentration after your sample was diluted into the assay tube. To calculate the concentration of your sample, use the following equation: Concentration of your sample = QF value × 200 x
Can a qubit be used as a broad range assay?
Below are protocols and downloadable assay files (.qbt) for Qubit fluorometers. Note that due to limitations of their optical systems, Qubit instruments cannot be used for DeNovix Broad Range assays. Limitations in sensitivity are outlined in the table below. Qubit® is a registered trademark of Thermo Fisher Scientific and its subsidiaries.
Can a qubit be used as a denovix assay?
DeNovix assays with user selected excitation sources are included in DeNovix EasyApps® Software for DS-11 FX and QFX instruments. Users of Qubit® fluorometers can also take advantage of the improved performance of DeNovix assays. Below are protocols and downloadable assay files (.qbt) for Qubit fluorometers.