What is Renilla luciferase used for?
The Renilla luciferase gene is commonly used as an internal control in luciferase-based reporter gene assays to normalize the values of the experimental reporter gene for variations that could be caused by transfection efficiency and sample handling.
What is TK Renilla vector?
The pRL Vectors are wild-type Renilla luciferase (Rluc) control reporter vectors. The HSV-thymidine kinase promoter (pRL-TK) is relatively weak and may be particularly useful in providing neutral constitutive expression of the Renilla luciferase control reporter.
What color is Renilla luciferase?
Renilla luciferase enzyme/protein (RL), purified from sea pansy (Renilla reniformis), is a bioluminescent soft coral that displays blue-green bioluminescence upon mechanical stimulation.
How does Gaussia luciferase work?
Gaussia luciferase can be expressed in mammalian cells using commercially available reporter plasmids. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate coelenterazine in a reaction that emits light (at 470 nm), and has considerable advantages over other reporter genes.
Can luciferase be scanned?
The bioluminescence emitted can be detected and amplified using specialized cameras with highly sensitive detection systems [18], revealing the sites and levels of luciferase expression and activity from within a living animal.
How long does luciferase stay in the body?
The most commonly used reporter for this purpose is a construct that can express firefly luciferase, normally a heat-unstable enzyme with a biological half-life of approximately 2 hours that must be continuously produced in order for imaging to be accomplished.
Why is luciferase the best reporter?
Luciferase-based assays are better than other reports because of the following advantages: Quick and real-time measurement. Exceptionally high sensitivity than fluorescent reporters like GFP (10- to 1,000 fold) Range of measurement is wide and dynamic.
Which protein is present in fireflies?
Luciferase
Luciferase from the firefly Photinus pyralis (EC 1.13. 12.7) is a peroxisomal protein found in the light-emitting organ known as the lantern within the abdomen of the insect. The firefly uses this enzyme to emit flashes of light to attract its mate.
Is Renilla luciferase secreted?
For example, Renilla luciferase is not secreted, and thus the Pierce Renilla-Firefly Luciferase Dual Reporter Assay Kit will not work for this type of time-course assay.
How is luciferin produced?
Luciferins typically undergo an enzyme-catalyzed reaction with molecular oxygen. The resulting transformation, which usually involves splitting off a molecular fragment, produces an excited state intermediate that emits light upon decaying to its ground state.
What’s the difference between luciferin and luciferase?
One is a luciferin, or a light-producing substance. The other is a luciferase, or an enzyme that catalyzes the reaction. In some cases, the luciferin is a protein known as a photoprotein, and the light-making process requires a charged ion to activate the reaction.
How is luciferase produced?
Luciferases can be produced in the lab through genetic engineering for a number of purposes. Luciferase genes can be synthesized and inserted into organisms or transfected into cells. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate.