How does the von Kossa stain work?
Von Kossa stain is widely used in histology to detect the presence of abnormal calcium deposits in the body. The principle of this coloration is based on the transformation of calcium salts into silver salts: calcium ions, bound to phosphates, are replaced by silver ions brought by a solution of silver nitrate.
How do you identify osteoblasts?
Osteoblasts are cuboidal mononuclear cells located on bone surfaces. Cytochemical methods to identify osteoblasts include toluidine blue stain (also known as tolonium chloride), alkaline phosphatase (ALP) enzymatic stain, immunochemical markers, and fluorescent protein reporters.
Which stain is used for bone?
Masson-Goldner trichrome staining is a commonly used method in bone histology and allows tissue identification by different coloring as well as by morphological identification.
Why is Histomorphometry important?
Histomorphometry plays an important role in monitoring changes in bone properties because of systemic skeletal diseases like osteoporosis and osteomalacia. Besides, quantitative evaluation plays an important role in fracture healing studies to explore the effect of biomaterial or drug treatment.
What type of stain is von Kossa?
Calcium Stain
The Von Kossa (Calcium Stain) is intended for use in the histological visualization of calcium deposits in paraffin sections.
Why is H and E staining used?
H and E staining helps identify different types of cells and tissues and provides important information about the pattern, shape, and structure of cells in a tissue sample. It is used to help diagnose diseases, such as cancer. Also called hematoxylin and eosin staining.
What organelles do osteoblasts have?
Osteoblasts have round mitochondria, in addition to lysosomes, vacuoles, and glycogen granules. These structural features of osteoblasts indicate that osteoblasts are robust.
How is bone histomorphometry performed?
Bone histomorphometry is a quantitative histological examination of an undecalcified bone biopsy performed to obtain quantitative information on bone remodeling and structure. Labeling agents taken before the procedure deposit at sites of bone formation allowing a dynamic analysis.
How is bone Histomorphometry done?
What are Trabeculae?
Trabecula: A partition which divides or partly divides a cavity. One of the strands of connective tissue projecting into an organ that constitutes part of the framework of the organ as, for example, the trabeculae of the spleen.
What is the Von Kossa staining method for calcium?
Description: This technique is for demonstrating deposits of calcium or calcium salt so it is not specific for the calcium ion itself. In this method, tissue sections are treated with a silver nitrate solution and the silver is deposited by replacing the calcium reduced by the strong light, and thereby visualized as metallic silver.
Who is the inventor of the Von Kossa stain?
History and method. The method was originally developed by von Kossa, and underwent several modifications. The stain principle is a precipitation reaction in which silver ions react with phosphate (not calcium) in the presence of acidic material Photochemical degradation of silver phosphate to silver then occurs under light illumination.
What kind of light is used for von Kossa?
The regular 60-100 watt light bulb usually gives weaker reaction so the calcium salts are often stained brown-black. Oxalate salts are usually believed to give a negative von Kossa staining. A negative control may be needed when there is any doubt that the resulting black deposits are calcium.