What is UPLC testing?
Impact Analytical offers Ultra-Performance Liquid Chromatography (UPLC) testing services which is the newest technology in liquid chromatography. UPLC involves the liquid chromatography at high pressures, providing chromatography that outperforms traditional HPLC in peak resolution.
What is a UPLC machine?
A: UPLC is Ultra Performance Liquid Chromatography. UPLC became the modern standard HPLC platform due to its power for increasing sample throughput, chromatographic efficiency, sensitivity and decreasing run time. UPLC system can handle pressure up to 15,000 psi.
What is the difference between UPLC and HPLC?
A: Both HPLC and UPLC are both liquid chromatography techniques used to separate the different components found in mixtures. However, UPLC operates at higher pressures (15,000 psi) and allows for lower particle sizes in columns, while HPLC operates at lower pressures (max <6000 psi).
Is UPLC better than HPLC?
The key difference between HPLC and UPLC here is that UPLC has a clear advantage of HPLC, thanks to its higher pressure. This allows for shorter run times, lower solvent consumption and greater analyte separation and detection by the detector.
How much does a HPLC test cost?
Capital costs are significant. You can get a “bare bones” HPLC for about $20K, but $40-50K is more typical by the time you throw in automation features and data analysis software.
Can I use HPLC column on UPLC?
Do not use the ACQUITY UPLC column in-line filter unit with an HPLC column. Set the high pressure limit of the ACQUITY Binary Solvent Manager to the maximum pressure recommended for the HPLC column.
How does a UPLC work?
UPLC cuts the mobile phase volume consumption by at least 80% compared to HPLC with a very short runtime (even less than 1 min). The smaller particles increase the pressure up to 1000 bars or more, which can alone increase the retention factor of the separation.
Can you use UPLC method for HPLC?
HPLC Method Transfer to UPLC – The HPLC method is transferred to a UPLC column with the same resolving power and stationary-phase chemistry, but smaller particle size, using the ACQUITY UPLC® Columns Calculator.
Why is HPLC test done?
HPLC is a sensitive and precise method for the identification of Hb A2, Hb F and abnormal haemoglobins. It has become the method of choice for thalassaemia screening because of its speed and reliability.
Why is ultra performance liquid chromatography ( UPLC ) so important?
For the speciality chemical and pharmaceutical industries, it is more important than ever to work efficiently, rapidly and to the highest quality levels. Ultra Performance Liquid Chromatography (UPLC) brings faster, more rapid method development and validation, more efficient LC quantitative analysis, and high-throughput QC analysis.
What is the purpose of the UPLC technology?
UPLC Technology facilitates improvements of resolution, sensitivity and speed to be achieved, without compromise.
What’s the difference between UPLC and HPLC pump pressure?
Whereas the pump pressure in HPLC is 40MPa, in UPLC this pressure can go up to 100MPa, which is what makes this technique so very exciting and efficient. Impact Analytical offers HPLC testing services to separate, characterize, identify, and quantitate semi- and non-volatile materials in various sample matrices.
Why do we need the ACQUITY UPLC system?
Whether the separation goal is to achieve ultra-fast analysis, increase throughput while maintaining resolution, improving resolution while decreasing analysis time or achieve ultra-high resolution, the flexibility of the ACQUITY UPLC System enables method requirements to be met [Figure 2].