What is fluorescein soluble in?
This product is soluble in 1 M NaOH (50 mg/ml), with heating. It is insoluble in water, benzene, chloroform, and ether. 6 It is also soluble in hot alcohol and glacial acetic acid solutions.
Does fluorescein dissolve in water?
It is not soluble in water or many organic solvents, but its disodium salt (fluorescein sodium, uranine, D & C Yellow No. 8) is highly water-soluble. Crystalline or powdered fluorescein is reddish orange, but in alkaline solution it has an intense yellow-green fluorescence.Farv
Is fluorescein oil soluble?
What Is Fluorescein? Abbey Color is one of a few companies in the world that makes and supplies fluorescein, which is an oil soluble dye.
What chemicals are used in fluorescein?
Fluorescein is an organic compound that has wide use as a synthetic coloring agent. It is prepared by heating phthalic anhydride and resorcinol over a zinc catalyst, and it crystallizes as a deep red powder.
How do you dilute fluorescein?
First, you may prepare a solution at 1mg/ml (2.6 mM) in molecular grade water, which you can then dilute to generate a 100 uM stock solution. Finally, we recommend preparing a working solution at 1 uM, which you can add directly to the PCR mix.M
How do you dissolve fluorescein?
Dilute the fluorescein solution by add- ing enough distilled or deionized water to reach the 500-mL mark on the beaker. Stir. Note: Prepare 1% fluorescein solution by dissolving 1 g of fluorescein in 100-mL distilled water.
Is fluorescein soluble in ethanol?
Soluble in hot ethanol, glacial acetic acid. Insoluble in water, benzene, chloroform, ether.Ordibe
Is Nile red water soluble?
Nile Red (compound A) fluoresces at about 530 nm with good quantum yields in apolar solvents. In more polar ones its fluorescence emission shows a dramatic, and potentially useful, shift to about 640 nm, but its quantum yield is significantly reduced. Further, Nile Red has a very poor solubility in aqueous media.
Does fluorescein react with bleach?
As mentioned before, Fluorescein will react to bleach, powder cleansers, and other cleaning materials, human and animal urine, certain types of metals, and/or any strong oxidizers. Fluorescein, after a period of time, will eventually oxidize reacting to the oxygen air, even if applied onto a sterile substrate.Ordibe
Do you dilute fluorescein?
Preparation For Administration Do not mix or dilute with other solutions or drugs. Flush intravenous cannulas before and after drugs are injected to avoid physical incompatibility reactions.
How does dilution affect fluorescence?
Excitation can be transfered by FRET to another fluorescein molecule. FRET is strongly dependent on the distance, therefore dilution can lead to stronger fluorescence.