What is pBluescript SK?
The pBC vectors were derived from the pBluescript II phagemid. Convenient selection when subcloning from ampicillin-resistant cloning vectors. With a large and versatile polylinker in Sac–>Kpn orientation and f1 origin in either (-) orientation.
Is pBluescript a cloning vector?
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
What is the insertional inactivation?
Insertional inactivation is a technique used in recombinant DNA technology. In this procedure, a bacteria carrying recombinant plasmids or a fragment of foreign DNA is made to insert into a restriction site inside a gene to resist antibiotics, hence causing the gene to turn non-functional or in an inactivated state.
How is insertional activation and blue white screening applied?
– Option a: In insertional inactivation, bacteria carrying recombinant plasmid is inserted at the restriction site in the gene. Therefore, white-colored colonies appear in the case of recombinant DNA and blue colored colonies will appear in the case of non-recombinant cells. Therefore, this option is correct.
Which is the standard cloning vector in pBluescript?
Plasmid Files Home» Resources» Plasmid Files» Basic Cloning Vectors» pBluescript SK(+) pBluescript SK(+) Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript KS(+)has a reversed MCS.
What can a pBluescript 2 phagemid be used for?
The pBluescript® II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
What’s the difference between pBluescript SK and KS?
Standard cloning vector (phagemid excised from lambda ZAP). The f1 (-) orientation allows rescue of antisense strand ssDNA. pBluescript SK (-) and pBluescript KS (-) differ by the orientation of the MCS.
Do you need to design two PCR primer sequences?
In order to do this, you will need to design two pcr primer sequences that will allow you to PCR amplify your gene of interest and at the same time append custom restriction recognition sequences on to either end of your gene.