What is a pGEX plasmid?

What is a pGEX plasmid?

Plasmid: pGEX-1 Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.

What is pGEX 2t?

Bacterial vector for expressing GST fusion proteins with a thrombin site.

What is a pET vector?

pET Bacterial Recombinant Protein Vector. Overview. The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.

What is thrombin cleavage?

A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein.

What is difference between plasmid and vector?

Main Difference – Plasmid vs Vector The main difference between plasmid and vectors is that plasmid is an extra-chromosomal element of mainly bacterial cells whereas vector is a vehicle that carries foreign DNA molecules into another cell. Plasmids can also be used as vectors.

How do you select an expression vector?

When you are choosing an expression vector, you’d better keep these things in mind:

1. Expression System. You need to choose an expression vector that is specific for the expression system.
2. Purification Tag.
3. Applications of the Target Protein.
4. Strength of the Promoter.
5. Selectable Marker.
6. Vector Size.

Is the pgex-4t1 vector available in Addgene?

This vector is NOT available from Addgene. thrombin or factor Xa protease sites to cleave protein from fusion. pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs. Hosts: E.coli. Related vectors: pGEX-2T.

Which is pGEX vectors provide all three translational reading frames?

Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site. pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from λgt11 libraries. 1. Kaelin, W.G. et al. Cell 70, 351 (1992).

Why do we use the pgex-6p expression vector?

The pGEX-6P Expression Vectors permit convenient site-specific cleavage and simultaneous purification on Glutathione Sepharose. The pGEX-6P series provides all three translational reading frames linked between the GST coding region and the multiple cloning site.

How many pGEX vectors are there in the world?

Thirteen pGEX vectors are available (see Figure). Nine of the vectors have an expanded multiple cloning site (MCS) that contains six restriction sites.