What is the purpose of the NanoDrop 2000 spectrophotometer?
Thermo Scientific NanoDrop 2000 and 2000c are full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure samples volumes as small as 0.5µL. .
How much is a NanoDrop spectrophotometer?
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How much is a NanoDrop 2000?
| Catalog Number | Mfr. No. | Price |
|---|---|---|
| Catalog Number | Mfr. No. | Price |
| SER2K3 Thermo Scientific™ Three-Year Extended Warranty for NanoDrop™ 2000/2000c viewProductCertificates | Thermo Scientific™ SER-2K-3 | Each for $4,592.65 |
Can I use NanoDrop for protein concentration?
The NanoDrop 2000c spectrophotometer can also be used to measure uncharacterized protein solutions, cell lysates, and crude protein extracts using colorimetric assays. Unlike the Protein A280 method, the Protein BCA method requires that a standard curve be generated before sample protein concentrations can be measured.
What is the advantage of using a NanoDrop spectrophotometer compared to using a regular spectrophotometer?
Why you would use a Nanodrop The main advantage the Nanodrop has over the Qubit is the ability to measure the purity of the sample. The 260/280 and 260/230 ratios give an indication of how pure the sample is from protein and salt contaminants.
How much can you put on NanoDrop?
Basically the nanodrop gives you the option to select DNA, RNA, Proteins. Yo need to select DNA , then place 2 μL of water (mili Q preferent) select “Blank” after that place another 2 μL of water to confirm that the measure is 0. Then place 2 μL of your sample. You will get the measurment.
How does NanoDrop quantify DNA?
If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample.
How are Thermo Scientific NanoDrop 2000 and 2000C used?
Thermo Scientific™ NanoDrop 2000 and 2000c are full-spectrum, UV-Vis spectrophotometers used to quantify and assess purity of DNA, RNA, Protein and more. The NanoDrop 2000 and 2000c are the only microvolume spectrophotometers with patented sample retention technology that measure sample volumes as small as 0.5μL.
How is spectrum recorded on NanoDrop 2000 / 2000C spectrophotometer?
When a NanoDrop 2000/2000c spectrophotometer is blanked, a spectrum is taken of the reference solution (blank) and stored in memory as an array of light intensities by wavelength. When a measurement of a sample is taken, the intensity of light that was transmitted through the sample is recorded.
Can You quantify proteins on a NanoDrop 2000C?
Yes, you can quantify proteins using the NanoDrop 2000/2000c Spectrophotometers. – Proteins may be quantified using a micro-volume sample on the pedestal (2000 and 2000c) or using a standard cuvette (2000c). Can I simply wipe the pedestal surface enough to prevent carryover on my NanoDrop 2000/2000c Spectrophotometer?
What are the spectrophotometers of the Thermo Scientific?
Thermo Scientific NanoDrop™ 2000/2000c Spectrophotometers measure 0.5- 2 ul samples with high accuracy and reproducibility. The NanoDrop 2000c model offers the convenience of both the NanoDrop patented sample retention technology and a traditional cuvette for sample measurements.