What is warthin Starry stain used for?
Warthin-Starry (WS) staining is an ancillary stain used in the detection of Helicobacter sp., spirochaete and other microorganisms in tissue sections. The present study aimed to determine the validity of WS stain in the confirmation of H. pylori diagnosis in gastric biopsies in comparison with anti-H.
Which organisms can be identified by impregnation staining?
Ilnportant bacteria identified by silver impregnation staining tech- niques include the spirochetes, Ccirnpylobncter; Helicobac- tet; Legionella, and others (Table 1).
What are histological stains?
There are various methods used to study tissue characteristics and microscopic structures of the cells. Histological staining is a series of technique processes undertaken in the preparation of sample tissues by staining using histological stains to aid in the microscope study (Anderson, 2011).
What is impregnation staining?
The Golgi silver impregnation technique is a simple histological procedure that reveals complete three-dimensional neuron morphology. This method is based in the formation of opaque intracellular deposits of silver chromate obtained by the reaction between potassium dichromate and silver nitrate (black reaction).
What is a silver impregnation stain?
Silver impregnation is the traditional method for detection of T. pallidum in formalin-fixed tissues. The Dieterle technique is believed to be more sensitive than the Warthin-Starry stain.
What is mild reticulin fibrosis?
The occurrence of mild fibrosis (defined as a loose network of reticulin fibers by EUMNET grading on myelofibrosis) is a common feature at diagnosis in these patients and does not correlate with specific clinical features.
What kind of stain is Warthin Starry stain?
Helicobacter pylori colonized on the surface of regenerative epithelium, stained with the Warthin-Starry method. Clusters of bacteria (arrow) shown on Warthin–Starry stain. The Warthin–Starry stain (WS) is a silver nitrate -based staining method (a silver stain) used in histology.
How to use Warthin Starry staining for spirochetes?
Warthin Starry Staining Technique For Spirochetes 1 Principle. This technique involves the argyrophilic reaction. The spirochetes are argyrophilic, which means they will… 2 Requirements. 3 Procedure. Deparaffinize and bring sections to distilled water. Place slides in 1% silver nitrate solution for 45… 4 Results and Interpretation. More
Can a Warthin Starry stain detect Bacillary peliosis?
The endothelial lining may be absent focally, especially during the formation of a lesion. 47 Warthin-Starry stain can detect Bartonella organisms in bacillary peliosis. 48 Hairy cell leukemia has an “Indian file” lymphoid infiltrate with abundant cytoplasm, creating a characteristic perinuclear halo.
How did Garvey modify the Steiner staining method?
Garvey modified Steiners method and was able to reduce the staining time required to perform the procedure. She used a new developing solution which is easier to prepare than Steiners original formula. The staining time was reduced more by Swisher who used a microwave oven in performing the method.