What is mtesr1?
mTeSR™1, now manufactured under cGMP, is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation.
How long does mTeSR last?
Note: If not used immediately, store complete mTeSR™ Plus medium at 2 – 8°C for up to 2 weeks. Alternatively, aliquot and store at -20°C for up to 6 months. Do not exceed the shelf life of the individual components. After thawing the aliquoted complete medium, use immediately or store at 2 – 8°C for up to 2 weeks.
Who invented stem cell technology?
Stem cells are undifferentiated cells that have the capacity to both differentiate and multiply into the 200 cells types that form a human being. In total, the human body is composed of an estimated 30 trillion cells, making stem cells extremely important to human development. Stem cells are also found…
Which is the best mtesr cell culture medium?
mTeSR™1 is a highly specialized, serum-free and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.
What kind of cells can MethoCult media be used for?
Select formulations can also be used with hematopoietic cells derived from human pluripotent stem cells (hPSCs) or from other species, specifically rats, non-human primates and dogs. Why Use MethoCult™ Media? STANDARDIZED AND QUALITY CONTROLLED.
How does mtesr plus maintain IPS and ES cells?
In addition, medium pH is more consistent during culture in mTeSR™ Plus. These modifications enhance the flexibility of the medium, allowing for greater ES and iPS cell expansion with daily feeding, while also maintaining cell quality during restricted feeding schedules such as every-other-day or weekend-free schedules.
What are the critical components of mTeSR1?
It is based on the mTeSR™1 formulation, with critical medium components such as fibroblast growth factor 2 (FGF2; also known as basic FGF [bFGF]) being stabilized in the “Plus” formulation. In addition, medium pH is more consistent during culture in mTeSR™ Plus.