How do I reconstitute M-CSF?
Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
What is M-CSF used for?
The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.
What is the difference between M-CSF and GM CSF?
M-CSF exhibits a mostly homeostatic expression pattern, whereas GM-CSF is a product of cells activated during inflammatory or pathologic conditions. Accordingly, M-CSF regulates the numbers of various tissue macrophage and monocyte populations without altering their “activation” status.
Where is M-CSF produced?
bone marrow
M-CSF is produced in the bone marrow and by endothelial cells, fibroblasts, and mononuclear phagocytes. Its effects are felt locally; that is, M-CSF is not found in the blood circulation.
Is M-CSF a cytokine?
Macrophage colony-stimulating factor (M-CSF) is a cytokine found in the brain whose receptor is expressed by microglia.
What produces M-CSF?
M-CSF is produced by osteoblasts and osteoblast precursors, but large amounts of M-CSF are also produced by osteocytes. M-CSF is present in two distinct biologically active forms, a membrane-bound and a secreted form, that result from alternative splicing of the CSF1 gene.
What is a GM-CSF injection?
Granulocyte-macrophage colony stimulating factor, also called GM-CSF, Sargramostim, or Leukine®, is a man-made form of a natural human hormone. It is a colorless liquid that is either injected under the skin or given by vein. GM-CSF helps the body make infection-fighting white blood cells, called neutrophils.
How does a colony-stimulating factors work?
Colony-stimulating factors (CSFs) are secreted glycoproteins that bind to receptor proteins on the surfaces of hematopoietic stem cells, thereby activating intracellular signaling pathways that can cause the cells to proliferate and differentiate into a specific kind of blood cell, usually white blood cells.
How does a colony stimulating factors work?
How does GM-CSF work?
GM-CSF is a monomeric glycoprotein that functions as a cytokine — it is a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.
Is GM-CSF proinflammatory?
GM-CSF was first characterized as a pro-inflammatory cytokine due to its ability to stimulate plasminogen-dependent fibrinolysis activity in mouse macrophages (Hamilton and others 1980).
Are CSFs cytokines?
Granulocyte-macrophage colony-stimulating factor, also known as GM-CSF and CSF2, is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages.