What is HindIII Lambda Digest?
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
What is EcoRI and HindIII?
Thermo Scientific Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.
Why lambda DNA is used in restriction digestion?
Since the lambda genome is approximately 48,000 base pairs, each restriction enzyme will cut the DNA several times and generate restriction fragments of different sizes. Each sample produces DNA fragments whose sizes can be estimated when run on an agarose gel using electrophoresis.
How many EcoRI sites are there in lambda DNA?
six recognition sites
ABOUT THIS PRODUCT: The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease.
What is λ HindIII used for?
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.
What is the HindIII lambda phage size marker?
125 to 23130 bp
Phage Lambda DNA/ Hind III Marker is used for determining the size of double-stranded DNA from 125 to 23130 bp.
What is HindIII used for?
Their primary function is to protect the host genome against invasion by foreign DNA, primarily bacteriophage DNA. There is also evidence that suggests the restriction enzymes may act alongside modification enzymes as selfish elements, or may be involved in genetic recombination and transposition.
Where did HindIII get name?
Restriction enzymes are named based on the organism in which they were discovered. For example, the enzyme Hind III was isolated from Haemophilus influenzae, strain Rd. The first three letters of the name are italicized because they abbreviate the genus and species names of the organism.
What is lambda DNA used for?
Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
What is digestion of DNA?
Restriction Digestion is the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE’s).
How many nucleotides does bacteriophage lambda?
The 12 nucleotide 5′ overhangs at the cos-ends of the linear Lambda DNA are the result of a cut by the enzyme terminase. This enzyme is encoded by Lambda itself and acts like a restriction enzyme during the replication of the phage DNA.
What is lambda phage DNA?
Lambda DNA, a linear, double-stranded phage DNA containing 12 bp single-stranded complementary 5′-ends, is derived from an Escherichia coli bacteriophage (Bacteriophage lambda cI857 Sam7). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
How is the HindIII digest of lambda DNA used?
The HindIII digest of lambda DNA (c I857 ind 1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
How to label lambda DNA / EcoRI plus HindIII marker?
Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow fragment or Klenow Fragment, exo- with the fill-in reaction ( see Resources for Protocols).
How many DNA fragments are in the HindIII digest?
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
How does Thermo Scientific purify lambda DNA?
Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme (s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker. Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase.