How do I claim compensation for FACSDiva?
Right-click the Cytometer Settings in the Browser and select Application Settings > Save. Select Experiment > Compensation Setup > Calculate Compensation. In the Single Stained dialog, select Link & Save. Right-click the Cytometer Settings in the Browser and select Save to Catalog.
What is FACSDiva?
BD FACSDiva™ Software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for today’s busy laboratory.
How do I export data from FACS Diva?
Choose File > Export > FCS files. Alternatively, right-click the selected item(s) and choose Export > FCS files. 3. Specify the FCS file version (use FCS 3.0) and parameters to export (use “linear” for all parameters).
How do you compensate FACS?
As a general rule, compensate with the fluorochromes from the far-red end of the spectrum (higher wavelength), step-wise down to those fluorochromes at the lower end of the spectrum (lower wavelength). Do not forget to check the compensation in all channels.
What is FACS analysis?
Fluorescence activated cell sorting (FACS) analysis is a derivative of flow cytometry that proceeds in a slightly different direction. The primary objective of FACS is to physically sort a heterogeneous cell sample into separate populations. Isolated cells can then be used for further research.
What is Biexponential transformation?
Biexponential scaling helps visualize data that is compressed against the low x- and y- axes. Specifically, they address the problem of how to visualize high dynamic range data that contains both negative and positive values. For a more general overview on transforms, go here.
What is PMT flow cytometry?
The most commonly used detectors for flow cytometry are the photomultiplier tubes (PMT) that reside in each channel of the instrument (Figure 1). In addition to converting the photons to photocurrent, the PMT amplifies the signal, a process that requires the application of a steady-state voltage to the detector.
How long does it take to get results from flow cytometry?
In addition, preliminary cytogenetic testing results are available within 24 hours and can be correlated with the flow cytometry result to provide the physician with a clearer understanding of the specific leukemia or lymphoma present.
What can flow cytometry detect?
Flow cytometry immunophenotyping may be performed on blood, bone marrow, or other samples to provide this additional information. It can detect normal cells as well as abnormal cells whose pattern of markers are typically seen with specific types of leukemia and lymphoma.
Who is the owner of BD facsdiva software?
BD FACSDiva software © Becton, Dickinson and Company. This software is the property of Becton, Dickinson and Company. Each sale of a stored unit of this software grants the purchaser a nontransferable, nonexclusive, personal license.
How to save time with facsdiva flow experiment?
Here are 8 time-saving FACSDiva tips to use the during your next flow experiment: 1. Compensation doesn’t require the ‘universal negative.’ It is important to remember that the background of the positive and negatives should be matched.
What does BD facsdiva software do for a cytometer?
BD FACSDiva™ Software provides a graphic of the cytometer’s physical optical configuration, showing the layout of detectors, filters and mirrors. This allows users to select the correct configuration for their work.
How big should the Diva database be for facsdiva?
Keep the Diva database clean. Diva is based in Java, and the database size can have a negative impact on the speed of the software. It’s best to keep the data base seize well under 15 gigabytes. This means you need to export your experiments on a regular basis.