Why is a pump required in HPLC?
An HPLC pump is also called a “solvent delivery system.” The purpose of the pump is to maintain a constant flow of mobile phase through the HPLC system. A standard HPLC pump can pump against back pressures up to 6,000 psi where a uHPLC pump typically exceeds 8,500 bar and can go as high as 20,000 psi or more.
Which column is used in HPLC?
The reversed-phase HPLC column is the most versatile and commonly used column type and can be used for a wide range of different types of analytes. Normal-phase HPLC columns have polar packing. The mobile phase is nonpolar and therefore usually an organic solvent such as hexane or methylene chloride.
What is dual piston pump?
A reciprocating pump design which produces a pressure differential across the pump on both the in and out stroke of each drive rod. Dual action pumps use two separate pumping chambers per drive rod. On the forward stroke, it pushes fluid out of the first chamber, and draws fluid into the other.
What is seal in HPLC?
The purpose of the seal wash solution is to remove buffer crystals so a pure organic solution of acetonitrile (ACN) should not be used alone. To dissolve the buffers, a suitable aqueous phase solution should be used (e.g. mixture with some organic such as 20% MeOH or ACN.
How do you use a dual action pump?
Connect the hose to the valve. Push down while turning clockwise until it won’t turn anymore. Insert the plug for pumping on both the down and up stroke. Pump with your knees to save wear on your arms.
What is slope in HPLC?
Slope tells the integrator how much baseline rise to expect. A high slope value means that peak starts will be marked later than a low slope value, all other things being equal.
What are the different types of HPLC pumps?
ICON Scientific offers three different HPLC pump designs: Single Piston Rapid Refill Pumps, Dual Piston Serial Pumps, and Dual Piston Parallel Pumps. Those designs can also be separated into two categories: Analytical Pumps and Preparative Pumps.
What is the back pressure of a HPLC pump?
This is accomplished regardless of the back pressure caused by the flow resistance of the HPLC column. A standard HPLC pump can pump against back pressures up to 6,000 psi where a uHPLC pump typically exceeds 8,500 bar and can go as high as 20,000 psi or more.
Which is Thermo Scientific UHPLC system for dual LC?
Double your throughput or deepen your sample knowledge using the Thermo Scientific Vanquish Duo UHPLC System for Dual LC, featuring a dual split sampler and two pumps, either as separate modules or combined in the dual pump.
What can a quaternary HPLC pump be used for?
Quaternary HPLC Pumps. These pumps are all about maximum flexibility and delivering stable peak retention times for the widest possible application range. Use this pump for excellent solvent proportioning required for fast, high-resolution separations; a range of HPLC/UHPLC applications and LC-MS applications.