What happened in AP lab 13?

What happened in AP lab 13?

In this lab we found that the boiling test tubes remained clear throughout the entire time interval. The room temperature test tubes had the fastest rate, reaching the number four on the color chart. By existing knowledge it is a known fact that high temperature environments denature proteins and their active sites.

What does high enzymatic activity mean?

Measuring Enzyme Function Therefore, a higher reaction rate indicates that the enzyme is catalyzing reactions more efficiently, whereas a lower rate means that it is working less efficiently. To determine the reaction rate, the reaction velocity is calculated by measuring the amount of product made per unit time.

What does enzyme activity indicate?

Enzyme activity is measured in units which indicate the rate of reaction catalysed by that enzyme expressed as micromoles of substrate transformed (or product formed) per minute.

Why was the color indicator guaiacol used?

In this experiment an indicator for oxygen will be used. The compound guaiacol has a high affinity for oxygen, and in solution, it binds instantly with oxygen to form tetraguaiacol, which is brownish in color. Using a color palette is a relative way to compare a change and is therefore qualitative.

What is the purpose of an enzyme lab?

The purpose of this lab is to investigate the impact of temperature, substrate concentration, enzyme concentration, and the presence of an inhibitor on the effectiveness and rate of an enzyme. If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well.

How is enzyme activity influenced by pH?

As pH increases, enzyme activity increases until it reaches an optimal point in which enzymes denatures and as pH increases, enzyme activity decreases. They can also interact with the active site and disrupt hydrogen binding and binding to ionic residues decreasing, or in some cases increasing, activity of the enzyme.

What is enzyme activity how is it controlled?

Enzymes can be regulated by other molecules that either increase or reduce their activity. Molecules that increase the activity of an enzyme are called activators, while molecules that decrease the activity of an enzyme are called inhibitors.

Why is enzymatic activity important?

Enzymes are proteins that help speed up chemical reactions in our bodies. Enzymes are essential for digestion, liver function and much more. Too much or too little of a certain enzyme can cause health problems. Enzymes in our blood can also help healthcare providers check for injuries and diseases.

Why enzyme activity assay is medically important?

Enzyme Assays. Several enzymes are important in clinical pathology. Enzymes characteristic of a tissue are released into the blood when the tissue is damaged; hence assays of serum enzyme levels can aid in the diagnosis or monitoring of specific diseases.

Begin typing your search term above and press enter to search. Press ESC to cancel.

Back To Top